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The effect of magnesium and vitamin E pre-treatments on irradiation-induced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study [Anatol J Cardiol]
Anatol J Cardiol. 2012; 12(6): 508-514 | DOI: 10.5152/akd.2012.159  

The effect of magnesium and vitamin E pre-treatments on irradiation-induced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study

Beste M. Atasoy1, Zerrin Özgen2, Korkut Bostancı3, Meral Yüksel4, Zeynep Özen1, Roman İbrahimov1, Ufuk Abacıoğlu1
1From Departments of Radiation Oncology, Marmara University, İstanbul-Turkey
2Clinic of Radiotherapy, Marmara University Pendik Education and Research Hospital, İstanbul-Turkey
3Thoracic Surgery Vocational School of Health Related Professions, Marmara University, İstanbul-Turkey
4Medical Laboratory, Vocational School of Health Related Professions, Marmara University, İstanbul-Turkey

Objective: The aim of this study was to investigate the effect of pre-treatment with the free radical scavenging molecules, magnesium and vitamin E, on lipid peroxidation to limit radiation-induced heart and lung injury. Methods: Female Sprague-Dawley rats were divided into 4 groups by a simple randomization method as saline-treated control (n=4), saline-treated irradiated (IR; n=6), magnesium sulphate-treated irradiation (IR) (Mg+IR; n=6) and vitamin E-treated IR (vit E+IR; n=6), respectively. The animals were given either saline, Mg (600mg/kg/day) or vit E (100 mg/kg/day) intraperitoneally for five days prior to irradiation. Twelve hours after the fifth injection, animals in irradiation groups were irradiated to 20 Gy using 6 MV photons in linear accelerator. Twenty-four hours later cardiac and lung tissue samples were obtained for determination of myeloperoxidase activity (MPO), malondialdehyde (MDA) levels, and luminol and lucigenin levels measured by chemiluminescence (CL) methods. Results: No significant changes were observed between cardiac and pulmonary MDA and CL results of the experimental groups. However, cardiac and pulmonary MPO activities in the saline-treated IR group were increased as compared to control group (p<0.05 for all), while in the Mg-pretreated and vit E pretreated groups neutrophil infiltration was reduced, reaching to statistical significance only in the Mg-pretreated group (p<0.05). Conclusion: Prophylactic use of magnesium sulfate has limited the infiltration of neutrophils to both the cardiac and pulmonary tissues at the early 24 h of irradiation. However, how limiting neutrophils as the sources of free radicals and inflammatory mediators would alter oxidative stress of heart and lung tissues in the long-term is not clear yet.

Keywords: Heart, irradiation, lung, magnesium, radioprotection, vitamin E


Beste M. Atasoy, Zerrin Özgen, Korkut Bostancı, Meral Yüksel, Zeynep Özen, Roman İbrahimov, Ufuk Abacıoğlu. The effect of magnesium and vitamin E pre-treatments on irradiation-induced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study. Anatol J Cardiol. 2012; 12(6): 508-514


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