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microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A [Anatol J Cardiol]
Anatol J Cardiol. 2018; 20(4): 198-205 | DOI: 10.14744/AnatolJCardiol.2018.98511  

microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A

Run- He Qin1, Hui Tao1, Shi- Hao Ni1, Peng Shi1, Chen Dai1, Kai- Hu Shi2
1Department of Cardiothoracic Surgery, The Second Hospital of Anhui Medical University; Anhui-China
2Department of Cardiothoracic Surgery, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine; Nanjing-China

Objective: This study aims to investigate the effect of miR-29a targeting the regulation of DNMT3A on the development of cardiac fibrosis in Sprague-Dawley (SD) rats.
Methods: In vivo experiment: SD rats were randomly divided into model and control groups. The cardiac and left ventricular indices in each group were calculated. The pathological changes of the myocardium were observed. The expression levels of miR-29a, CollA1, α-SMA, and DNMT3A in the myocardium of each group were detected. In vitro experiment: The cardiac fibroblasts (CFs) of SD rats were isolated from the myocardial tissue of SD rats and cultured. The miR-29a mimics, inhibitors, DNMT3A-siRNA, and control-siRNA were transfected into CFs. The expression levels of miR-29a, DNMT3A, CollA1, and α-SMA were detected, and the proliferation of CFs after transfection was observed.
Results: The heart weight index of the rats in the model group increased significantly compared with that in the control group. Obvious collagen deposition was observed in the myocardial tissue of the model group. The expression levels of CollA1, α-SMA, and DNMT3A in the model group were significantly higher than those in the control group (p<0.05).
Conclusion: miR-29a reduced the activation and proliferation of CFs to improve cardiac fibrosis probably by the downregulation of DNMT3A.

Keywords: miR-29a, DNMT3A, cardiac fibroblasts, cardiac fibrosis


Run- He Qin, Hui Tao, Shi- Hao Ni, Peng Shi, Chen Dai, Kai- Hu Shi. microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A. Anatol J Cardiol. 2018; 20(4): 198-205

Corresponding Author: Kai- Hu Shi, China


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