ISSN 2149-2263 | E-ISSN 2149-2271
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The effects of acute and intermittent hypoxia on the expressions of HIF-1? and VEGF in the left and right ventricles of the rabbit heart [Anatol J Cardiol]
Anatol J Cardiol. 2011; 11(5): 379-385 | DOI: 10.5152/akd.2011.104

The effects of acute and intermittent hypoxia on the expressions of HIF-1? and VEGF in the left and right ventricles of the rabbit heart

Demet Tekin1, Ali Doğan Dursun1, Metin Baştuğ1, Gökhan Karaorman1, Hakan Fıçıcılar1
Department of Physiology, Faculty of Medicine, Ankara University, Ankara-Turkey

Objective: Hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) are involved in signaling mechanisms of cellular responses to hypoxia. These factors have been investigated in tissue samples by simulating different altitudes by changing the percentage of oxygen. We aimed first to evaluate the effect of normobaric, systemic hypoxia (11% O2) on HIF-1α and VEGF mRNA levels in the heart muscle; secondly, to compare the levels of HIF-1α and VEGF mRNA in the left and right ventricle muscles. Methods: In this experimental study, 33 New Zealand male rabbits were assigned to control, acute hypoxia (4 hours) and intermittent hypoxia (4 hours/day for 14 days) groups (n=11/group). Total RNA was isolated from right and left ventricles of the heart. The expressions of HIF-1α and VEGF mRNAs were investigated by using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. The obtained data were compared by using ANOVA and paired t-test. Results: The results indicated that left ventricle VEGF mRNA expressions in both acute and intermittent hypoxia groups (1.08±0.15 and 1.03±0.19, respectively) were higher than that in the control group (0.88±0.15) (p=0.03). Hypoxia treatments did not significantly alter HIF-1α mRNA in both ventricles (p=0.60 and p=0.51 for left and right ventricles, respectively). Conclusion: Since systemic hypoxia results in induction of VEGF mRNA up-regulation only in left ventricle, it could be related to its higher metabolic activity and oxygen utilization. Hypoxia induced changes in the expression of HIF-1α mRNA may not be the only determining factor for HIF-1/VEGF pathway induction or the observed VEGF induction could be through other hypoxia sensitive pathways.

Keywords: Acute hypoxia, intermittent hypoxia, expression, heart, HIF-1?, rabbit, VEGF, ventricle

Demet Tekin, Ali Doğan Dursun, Metin Baştuğ, Gökhan Karaorman, Hakan Fıçıcılar. The effects of acute and intermittent hypoxia on the expressions of HIF-1? and VEGF in the left and right ventricles of the rabbit heart. Anatol J Cardiol. 2011; 11(5): 379-385
Manuscript Language: English


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