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Effects of Nigella sativa L. seed oil on intima–media thickness and Bax and Caspase 3 expression in diabetic rat aorta [Anatol J Cardiol]
Anatol J Cardiol. 2016; 16(7): 460-466 | DOI: 10.5152/AnatolJCardiol.2015.6326  

Effects of Nigella sativa L. seed oil on intima–media thickness and Bax and Caspase 3 expression in diabetic rat aorta

Gökhan Cüce1, Mehmet Enes Sözen1, Seda Çetinkaya2, Halime Tuba Canbaz1, Hatice Seflek1, Serpil Kalkan1
1Department Of Histology And Embryology, Faculty Of Meram Medicine, Necmettin Erbakan University; Konya-turkey
2Department Of Histology And Embryology, Faculty Of Medicine, University Of Kto Karatay University; Konya-turkey

Objective: Hyperglycaemia is an important risk factor for the development and progression of the macrovascular and microvascular complications that occur in diabetes. The expression of apoptotic markers in the aortic medial layer of diabetic rats and the effects of N. sativa L. seed oil on the expression of these markers were investigated in this study.
Methods: Four-month-old adult female Wistar rats (n=21) were divided into 3 groups: Group 1, control; Group 2, diabetes and Group 3, diabetes+N. sativa L. seed oil. Group 3 received 0.2 mg/kg/day N. sativa L. seed (black cumin) oil intraperitoneally 6 days per week for 30 days. At the end of the experiment, abdominal and thoracic aortas of all animals were collected and fixed in 10% formalin solution. Then, 5-μm-thick sections were stained with Verhoeff–Van Gieson stain to evaluate Bax and Caspase 3 expression. Tunica intima–media thickness was measured using the stained sections.
Results: There were no significant differences in abdominal or thoracic aortic intima–media thickness among the 3 groups. However, there were significant differences in Bax and Caspase 3 expression in the tunica media of the thoracic and abdominal aortas between Group 1 and Group 2 (p<0.05) and between Group 2 and Group 3 (p<0.05) evaluated with the Kruskal–Wallis and Mann–Whitney U tests.
Conclusion: It is understood that N. sativa L. seed oil is effective against diabetes. N. sativa L. seed oil is a plant material and has value for further investigation to develop diabetes treatment strategies for preventing apoptosis in vascular structures. (Anatol J Cardiol 2016; 16: 460-6)

Keywords: diabetes, aorta, Nigella sativa L. seed oil, Bax, Caspase 3


Gökhan Cüce, Mehmet Enes Sözen, Seda Çetinkaya, Halime Tuba Canbaz, Hatice Seflek, Serpil Kalkan. Effects of Nigella sativa L. seed oil on intima–media thickness and Bax and Caspase 3 expression in diabetic rat aorta. Anatol J Cardiol. 2016; 16(7): 460-466

Corresponding Author: Gökhan Cüce, Türkiye


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