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G protein β3 subunit gene polymorphism in Turkish hypertensives [Anatol J Cardiol]
Anatol J Cardiol. 2008; 8(5): 331-335

G protein β3 subunit gene polymorphism in Turkish hypertensives

Emin Alioğlu1, Ertuğrul Ercan2, İstemihan Tengiz1, Ahmet Yıldız3, Uğur Önsel Türk1, Serkan Saygı4, F. Sırrı Çam5, Afig Berdeli6
1Department of Cardiology, Central Hospital, İzmir
2Department of Cardiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale,
3Department of Cardiology, Gazi Hospital, İzmir,
4Department of Cardiology, Karşıyaka State Hospital, İzmir,
5Department of Medical Biology and Genetics, Faculty of Medicine, Celal Bayar University, Manisa,
6Department of Pediatrics, Faculty of Medicine, Ege University, İzmir, Turkey

Objective: G protein is one of the most important regulators of intracellular signaling pathways. C825T polymorphism of G protein β3 subunit is associated with increased intracellular signal transduction. The 825T allele has been found associated with a variety of cardiovascular risk factors, including hypertension. The aim of the present study was to investigate the association between the C825T polymorphism of the G protein β3 subunit and essential hypertension in Turkish population.
Methods: This cross-sectional, case-controlled study included 209 patients with essential hypertension (Patient group) and 82 subjects with normal blood pressure (Control group). The G protein β3 subunit C825T gene polymorphism was determined by polymerase chain reaction. Hypertension was defined according to JNC VII criteria. Statistical analysis was performed using Chi square and unpaired t tests. Logistic regression analysis was used to study association between hypertension and genotypes.
Results: We found that the frequencies of the G protein β3 subunit C825T polymorphism in hypertensive and control groups were 17.7%, 59.3%, 23.0% and 32.9%, 48.8%, 18.3%, (CC, CT, TT) respectively (χ2=7.963, p=0.019). In the multivariate logistic regression analysis CT genotype had 2.2 (OR=2.262, 95% CI 1.228-4.167, p=0.009), and TT genotype had 2.3 times (OR=2.335, 95% CI 1.089-5.008, p=0.029) greater risk of hypertension compared to CC genotype.
Conclusion: It seems that the G protein β3 subunit C825T gene polymorphism is associated with systolic and diastolic blood pressure. Furthermore, the study indicates that the G protein β3 subunit may be a susceptible gene to essential hypertension.

Keywords: Hypertension, G protein, gene, polymorphism, genetics, predictive models, logistic regression analysis


Hipertansif Türklerde G protein β3 subunit gen polimorfizmi

Emin Alioğlu1, Ertuğrul Ercan2, İstemihan Tengiz1, Ahmet Yıldız3, Uğur Önsel Türk1, Serkan Saygı4, F. Sırrı Çam5, Afig Berdeli6
1Department of Cardiology, Central Hospital, İzmir
2Department of Cardiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale,
3Department of Cardiology, Gazi Hospital, İzmir,
4Department of Cardiology, Karşıyaka State Hospital, İzmir,
5Department of Medical Biology and Genetics, Faculty of Medicine, Celal Bayar University, Manisa,
6Department of Pediatrics, Faculty of Medicine, Ege University, İzmir, Turkey

Amaç: G protein sistemi intrasellüler sinyal iletimi yolaklarının, en önemli düzenleyicilerinden birisidir. G protein β3 subunitindeki C825T polimorfizmi intrasellüler sinyal transdüksiyonundaki artış ile ilişkilidir. C825T alleli ile hipertansiyonu da içeren çeşitli kardiyovasküler risk faktörleri arasındaki ilişki bilinmektedir. Bu çalışmada amacımız, Türk toplumunda C825T polimorfizmi ile hipertansiyon arasındaki ilişkiyi araştırmaktır.
Yöntemler: Vaka-kontrollü, enine-kesitsel çalışmaya esansiyel hipertansiyonu olan 209 hasta (hasta grubu) ve kan basıncı normal olan 82 birey (kontrol grubu) alınmıştır. G protein β3 subunitindeki C825T polimorfizmi polimeraz zincir reaksiyonu ile belirlendi. Hipertansiyon tanısı JNC VII kriterlerine göre konuldu. istatistiksel analiz Ki-kare, ve eşleştirilmemiş t testi ile yapıldı. Hipertansiyon ile genotip arasında ilişki lojistik regresyon analiz ile incelendi.
Bulgular: G protein β3 subunitindeki C825T polimorfizmi frekansı (CC, CT, TT) hipertansif grupta ve kontrol grubunda sırası ile %17.7, %59.3, %23.0 ve %32.9, %48.8, %18.3, olarak saptandı (χ2=7.963, p=0.019). Lojistik regresyon analizinde CC genotipine göre CT (OR=2.262 (%95GA 1.228-4.167, p=0.009) ve TT (OR=2.335, %95GA 1.089-5.008, p=0.029) genotiplerinde hipertansiyon olma riski sırası ile 2.2 ve 2.3 kat olarak artmış olarak hesaplanmıştır.
Sonuç: Mevcut bulgular, Türk toplumunda G protein β3 subunitindeki C825T polimorfizmi ile sistolik ve diyastolik kan basıncı arasında bir ilişki söz konusu olduğunu göstermektedir. Ayrıca, bu çalışmada esansiyel hipertansiyona duyarlılık geni, G protein β3 subunitindeki C825T polimorfizmi olabileceği gösterilmiştir.

Anahtar Kelimeler: Hipertansiyon, G protein, polimorfizm, genetik, prediktif modeller, lojistik regresyon analizi


Emin Alioğlu, Ertuğrul Ercan, İstemihan Tengiz, Ahmet Yıldız, Uğur Önsel Türk, Serkan Saygı, F. Sırrı Çam, Afig Berdeli. G protein β3 subunit gene polymorphism in Turkish hypertensives. Anatol J Cardiol. 2008; 8(5): 331-335

Corresponding Author: Ertuğrul Ercan, Türkiye


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